Stages of Embryo Development During In-Vitro Fertilization (IVF)
Stages of IVF Embryo Development
At Arizona Center for Fertility Studies, all embryology laboratory equipment, incubators and ancillary Assisted Reproductive Technology (ART) equipment has back-up electrical power in case of a power outage.
Fertilization occurs in 4-6 hours in humans but there are no visible signs until approximately 17-18 hours later. The first signs that fertilization have occurred visibly in the development of two round bodies in the center of the egg. The slightly smaller body is the female pronucleus and contains 23 chromosomes that the egg contributes to the embryo; the other round body is the male pronucleus and contains the contribution of 23 chromosomes from the sperm. It is critical that the egg is checked at this point in time for fertilization, because over the next 6 hours or so, the two pronuclei come together in a process known as syngamy, where the two pronuclei join chromosomes, forming one nucleus of 46 chromosomes.
Within the next 6 hours (30 hours from fertilization) the, now "fertilized egg" will divide producing a 2-cell embryo. Further division or "cleavage" takes place every 10-12 hours, producing a 4- cell embryo on day 2, an 8-cell embryo on day 3, a morula or ball of, too many cells to count, on day 4, and a blastocyst on day 5.
Graphic representation of the different stages of embryo development from either the release of an egg at ovulation or recovery of eggs during In-Vitro Fertilization (IVF), to fertilization inside the fallopian tube or outside the body, by ICSI, to the different stages of embryo development in the fallopian tube or in the Assisted Reproductive Technology (ART) incubator prior to embryo transfer at the 8-cell stage or blastocyst stage
After fertilization, if the embryo is looked at too late in this process, than "abnormal" fertilization can be overlooked and the embryo will "appear normal". This "abnormal" embryo can divide and even implant but will not produce a viable pregnancy and is only destined to abort, resulting in a miscarriage and disappointment.
Fertilized Pronuclear Embryo
A fertilized pronuclear embryo showing syngamy, or coming together, of the male and female pronuclei at about 24 hours.
Fertilized 2 Cell Embryo
A normal fertilized 2-cell embryo at about 30 hours from egg recovery.
Fertilized 4 Cell Embryo
A normal 4-cell embryo on day 2 approximately 48 hours after fertilization.
Fertilized 8 Cell Embryo
A normal 8-cell embryo on day 3 approximately 72 hours after egg recovery.
A normal morula on day 4 showing signs of compaction and having too many cells to count.
A perfectly normal looking day 5 blastocyst with an ICM (stem cells where the baby develops from), blastocyst cavity (C) and an outer wall of trophoblastic cells (where the placenta develops from).